刘宁, 梁寒, 李强, 李慧, 任秀宝, 张毓青, 张汝鹏, 刘勇, 王晓娜, 丁学伟, 潘源, 邓靖宇, 詹宏杰, 郝希山. MAGE-1 MAGE-3及AFP基因在肝癌组织中的表达[J]. 中国肿瘤临床, 2007, 34(9): 493-496.
引用本文: 刘宁, 梁寒, 李强, 李慧, 任秀宝, 张毓青, 张汝鹏, 刘勇, 王晓娜, 丁学伟, 潘源, 邓靖宇, 詹宏杰, 郝希山. MAGE-1 MAGE-3及AFP基因在肝癌组织中的表达[J]. 中国肿瘤临床, 2007, 34(9): 493-496.
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Liu Ning, Liang Han, Li Qiang et al, . The Expression of MAGE- 1, MAGE- 3 and AFP mRNA in Hepatocellar Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(9): 493-496.
Citation: Liu Ning, Liang Han, Li Qiang et al, . The Expression of MAGE- 1, MAGE- 3 and AFP mRNA in Hepatocellar Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(9): 493-496.
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MAGE-1 MAGE-3及AFP基因在肝癌组织中的表达

The Expression of MAGE- 1, MAGE- 3 and AFP mRNA in Hepatocellar Carcinoma

    摘要
  • 摘要: 目的:探讨MAGE-1、MAGE-3和AFP mRNA在肝癌组织中的表达及用于检测微小转移的可能性。方法:应用聚合酶链反应(PCR)检测86例原发性肝癌患者肝癌组织中MAGE-1、MAGE-3和AFP mRNA的表达。结果:86例原发性肝癌患者肝癌组织中MAGE-1、MAGE-3和AFP mRNA的阳性率分别为34.9%(30/86)、60.5%(52/86)和69.8%(60/86);所有患者肝癌组织至少表达其中一种mRNA。结论:MAGE-1、MAGE-3结合AFP mRNA在肝癌组织中表达率较高,有可能用于联合检测肝癌血行微小转移。

     

    Abstract: Objective: To investigate the expression of MAGE- 1, MAGE- 3 and AFP mRNA in tumor tissue from patients with hepatocellular carcinoma (HCC) to develop a multiple marker reverse transcriptase polymerase chain reaction (RT- PCR) assay for detection of micrometastasis in circulation.Methods: Tumor tissue specimens were obtained from 86 patients with hepatocellular carcinoma and fluorescent quantitative RT- PCR was used to detect the MAGE- 1, MAGE- 3, and AFP mRNA in the specimens. Results: In 86 tumor specimens, the positivity for the MAGE- 1, MAGE- 3, and AFP genes was 34.9% (30/86), 60.5% (52/86) and 69.8% (60/86), respectively, and all specimens expressed at least one marker. Conclusion: Our results indicate that a multimarker RT- PCR assay with cancer-specific markers including MAGE- 1 and MAGE- 3 in combination with a liver- specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.

     

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