Abstract: Objective: To discuss the influence of tumor osseous metastasis on the patients receiving autologous peripheral blood stem cell collection. Methods: A total of 36 patients with malignant diseases received the autologous peripheral blood stem cell transplantation, during a period from April 2004 to June 2006, were chosen. The patients were divided into two groups, i.e. the group A for patients with a complication of tumor osseous metastasis and group B without the metastasis. Both groups were administrated with Taxotere 120mg/m2 plus granulocyte colony- stimulating factor(G- CSF) 5μ g/kg/d, the mobilization regimen. Blood cell separator was used to collect the mononuclearcells. The proportion of harvested CD34+ cells in peripheral blood, the collected monoclearcells were detected by Flow Cytometer and the numbers of CD34+ cells calculated accordingly to appraise the difference between effect of the mobilization and quality of the collections of the two groups. Results: After the mobilization in the group A and B, the number of the peripheral blood mononuclear cells (PBMC) was 39.3±14.7% and 41.1± 12.4% and the proportion of CD34+ cells was 0.16± 0.07% and 0.17± 0.10%, respectively. After the tests, there was no significant difference between the number of harvested PBMC and CD34+ cells of the two groups, i.e., 3.47± 1.16× 108/kg and 2.52± 1.43× 106/Kg in group A and 4.02±1.31× 108/kg and 2.73± 1.87× 106/Kg in group B, respectively. Conclusion: Osseous metastasis, as a single factor, may have no impact on mobilization and harvesting of the hematopoietic stem cells and their engraftment after autotransplantation.