Abstract: Objective: To investigate the effect of bradykinin on the expression of interleukin-6(IL-6) in C6 glioma cells. Methods: Semi-quantitative RT-PCR and radioimmunoassay were used to detect the expression of IL-6 mRNA in C6 glioma cells and IL-6 protein in culture medium from cells treated with 1μ mol/L bradykinin, respectively. Results: Semi-quantitative RT-PCR revealed that IL-6mRNA was detected at different time points (0, 5, 10, 15, 30 and 60 min) in C6 glioma cells after treatment with bradykinin at 1μ mol/L, but there was no significant difference between the treatment group and the control group (P>0.05). Radioimmunoassay revealed that expression of IL- 6 protein wasnot detected in the cell culture medium. Conclusion: There is a close correlation between interleukin-6 (IL-6) expression and malignant proliferation in glioma. Bradykinin fails to evoke an enhancement inthe expression of IL-6 in C6 glioma cells within an hour, which may provide a favorable experimental basis for the clinical application of bradykinin.