李晓丽, 彭利, 张萌, 徐卓, 王顺祥, 唐瑞峰, 张凤瑞. Survivin反义寡核苷酸对肝癌细胞增殖和凋亡的影响[J]. 中国肿瘤临床, 2007, 34(16): 917-920.
引用本文: 李晓丽, 彭利, 张萌, 徐卓, 王顺祥, 唐瑞峰, 张凤瑞. Survivin反义寡核苷酸对肝癌细胞增殖和凋亡的影响[J]. 中国肿瘤临床, 2007, 34(16): 917-920.
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Li Xiaoli, Peng Li, Zhang Meng et al, . Effect of Antisurvivin Oligonucleotides on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(16): 917-920.
Citation: Li Xiaoli, Peng Li, Zhang Meng et al, . Effect of Antisurvivin Oligonucleotides on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(16): 917-920.
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Survivin反义寡核苷酸对肝癌细胞增殖和凋亡的影响

Effect of Antisurvivin Oligonucleotides on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell

    摘要
  • 摘要: 目的:观察Survivin反义寡核苷酸对SMMC-7721细胞增殖、凋亡的影响。方法:设计合成特异性Survivin反义寡核苷酸,转染肝癌SMMC-7721细胞,MTT法测定Survivin ASODN对细胞增殖抑制情况的影响,FCM法检测对细胞周期、凋亡及Survivin蛋白表达的影响。结果:Survivin ASODN可抑制SMMC-7721细胞的生长增殖,并呈浓度和时间依赖性。ASODN转染组可诱导SMMC-7721细胞凋亡(P<0.01),细胞周期阻滞于G2/M期(P<0.05)。ASODN转染组Survivin蛋白表达水平显著降低(P<0.01)。结论:Survivin ASODN能下调SMMC-7721细胞Survivin表达,并可抑制其增殖并诱导凋亡。

     

    Abstract: Objective: To investigate the effect of antisense oligonucleotide(ASODN) targeting sur-vivin on apoptosis and proliferation of the SMMC-7721 hepatocellular carcinoma cell line. Methods:ASODN targeting survivin was designed, constructed and transfected to SMMC-7721 cells by oligofec-tamine. Inhibitory action of the cell growth was measured using MTT assay. Cell cycle, apoptosis and the expressions of survivin protein were detected by flow cytometry. Results: Treatment of with survivin ASODN suppressed cell proliferation significantly in both the time-dependent and the dose-dependent manner. The ASODN groups significantly induced apoptosis (P<0.01) and increased the proportion ofcells in the G2/M phase (P<0.05). The expression of survivin protein were significantly decreased com-pared with that of control group (P<0.01). Conclusions: The expressions of survivin in SMMC-7721cell are downregulated by survivin ASODN. ASODN survivin can inhibit the proliferation of the SMMC-7721 hepatocellular carcinoma cells and induce apoptosis.

     

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