bFGF和celecoxib对胃癌BGC-823细胞株生长的影响及其机制

聂海祺; 孙黎光; 叶丽平

bFGF和celecoxib对胃癌BGC-823细胞株生长的影响及其机制

Effects of bFGF and Celecoxib on Growth in BGC-823 Gastric Carcinoma Cells

摘要

摘要: 目的 :探讨bFGF和celecoxib对人胃癌BGC～823细胞增殖及凋亡的作用及对COX-2、NF-κB、VEGF蛋白表达的影响。方法 :bFGF和celecoxib作用于BGC-823人胃癌细胞后,MTT比色法测定细胞的增殖,使用流式细胞仪检测细胞凋亡率,荧光显微镜观察细胞凋亡形态,应用Western印迹分析法检测COX-2、NF-κB和VEGF蛋白的表达。结果 :25ng/ml bFGF对BGC-823细胞有促增殖作用,能增强COX-2、NF-κB、VEGF蛋白表达,呈时间依赖性(P<0.01)。不同浓度的celecoxib对BGC-823人胃癌细胞均有增殖抑制作用,并能促进细胞凋亡,使COX-2、NF-κB、VEGF蛋白表达明显减弱,呈浓度依赖性(P<0.01)。结论 :bFGF对BGC-823细胞具有促进增殖和COX-2、NF-κB、VEGF蛋白表达的作用。celecoxib对BGC-823细胞具有促进凋亡和抑制COX-2、NF-κB、VEGF蛋白表达的作用。COX-2、NF-κB、VEGF之间具有相关性(r分别为0.852、0.908、0.930,P<0.01)。

Abstract: Objective : To investigate the effects of bFGF and celecoxib on proliferation, apoptosis and expression of COX-2, NF-kB, and VEGF protein in BGC-823 gastric carcinoma cells. Methods : BGC-823 gastric carcinoma cells were treated with bFGF and celecoxib. Proliferation, apoptosis, mor-phology and expression of COX-2, NF-kB, and VEGF protein were observed using MTT, flow cytome-try, fluorescence microscopy and Western blotting, respectively. Results : bFGF promoted BGC-823 cell proliferation and expression of COX-2, NF-kB and VEGF protein at a concentration of 25 ng/ml in a time-dependent manner (尸<0.01). Celecoxib at different concentrations inhibited proliferation of BGC- 823 cells and induced apoptosis. The expression of COX-2, NF-kB and VEGF protein was attenuated in a dose-dependent manner (P<0.01). Conclusion : bFGF can promote BGC-823 cell proliferation and expression of COX-2，NF-kB, and VEGF protein. Celecoxib promotes apoptosis of BGC-823 cells and inhibits expression of COX-2, NF-kB and VEGF protein in a dose-dependent manner. COX-2, NF- kB and VEGF show interaction during the progression of carcinoma (r=0.852, 0.908 and 0.930，respec-tively; P<0.01).

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