Abstract: Objective : To explore the effect of RhoA subfamily members, i.e., RhoA, RhoB and RhoC, on the malignant phenotype of gastric cancer cells. Methods : The vectors of pCEFL-GST/V14RhoA, pCEFL-GST/wt-RhoB and pCEFL-GST/wt-RhoC were transfected into human gastric cancer cell SGC7901 by lipofectamine2000, respectively. Then the malignant biological behaviors of the transfected cells were explored. Results : The enhancement of RhoA activity can promote the proliferation of gastric cancer cell and can reduce the sensitivity of anticancer drugs; Up-regulation of RhoB can restrain the growth rate of gastric cancer cell and can enhance the sensitivity of anticancer drugs. The up-regulation of RhoC did not affect the proliferation and sensitivity of the anticancer drugs, but can promote the migration of gastric cancer cells. Conclusion : RhoA subfamily members can play an important but different role in regulating malignant behaviors of gastric cancer cells.