鞠培新, 石文君. 三氧化二砷在诱导非小细胞肺癌NCI-H157细胞凋亡过程中对Survivin基因表达Caspase-3的影响[J]. 中国肿瘤临床, 2007, 34(19): 1097-1100.
引用本文: 鞠培新, 石文君. 三氧化二砷在诱导非小细胞肺癌NCI-H157细胞凋亡过程中对Survivin基因表达Caspase-3的影响[J]. 中国肿瘤临床, 2007, 34(19): 1097-1100.
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Ju Peixin, Shi Wenjun. The Impact of As2O3 on Expression of Survivin and Caspase-3 in the Process of Inducing NCI-H157 cell Apoptosis in Non-Small Cell Lung Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(19): 1097-1100.
Citation: Ju Peixin, Shi Wenjun. The Impact of As2O3 on Expression of Survivin and Caspase-3 in the Process of Inducing NCI-H157 cell Apoptosis in Non-Small Cell Lung Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2007, 34(19): 1097-1100.
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三氧化二砷在诱导非小细胞肺癌NCI-H157细胞凋亡过程中对Survivin基因表达Caspase-3的影响

The Impact of As2O3 on Expression of Survivin and Caspase-3 in the Process of Inducing NCI-H157 cell Apoptosis in Non-Small Cell Lung Cancer

    摘要
  • 摘要: 目的 :研究三氧化二砷(As2O3)在诱导非小细胞肺癌NCI-H157凋亡过程中对Survivin基因表达及Caspase-3的影响,探讨As2O3诱导肿瘤细胞凋亡的作用机理研究。 方法 :首先对NCI-H157细胞培养和增殖测定,然后采用RT-PCR法检测Survivin mRNA表达;Western Blot检测Survivin及Caspase-3蛋白。 结果 :RT-PCR结果显示,10μmol/L As2O3作用于NCI-H157细胞12h,24h,48h同对照组相比,Survivin mRNA表达分别降至85.7%,59.4%,22.5%,处理72h后几乎检测不到Survivin mRNA的表达。Western Blot解析结果显示,Survivin蛋白表达在5μmol/L As2O3处理后分别下调至75.2%(12h),54.3%(24h),37.5%(48h)及20.3%(72h)。分别用不同浓度的As2O3 5～20μmol/L作用NCI-H157细胞24h,Survivin蛋白表达随着As2O3浓度的增加逐渐下降。As2O3作用12h之内只能检测到非激活状态32KD的proCaspase-3,处理24h后出现17KD的活性亚基,直至72h。 结论 :As2O3以时间、剂量依赖的方式抑制NCI-H157细胞增殖,诱导细胞凋亡过程中下调Survivin mRNA及蛋白表达,并活化Caspases-3,提示As2O3诱导的NCI-H157细胞凋亡机制可能与下调Survivin基因表达及激活Caspase-3活性有关。

     

    Abstract: Objective :To examine the expression of survivin and casepase-3 in As2O3-inducedapoptosis and investigate the mechanisms of apoptosis-inducing effect of As2O3 on NCI-H157 cells. Methods :Survivin mRNA expression was detected by semi-quantitative RT-PCR. Survivin and cas-pase-3 were analyzed by Western blot. Results :Apoptosis of NCI-H157 cells was induced by As2O3 atconcentrations of 10μ mol/L and above. Compared with the control group, the survivin RNA expressionrate in 10μ mol/L As2O3 treatment group after 12h, 24h and 48h was 85.7%, 59.4%, and 22.5%, re-spectively, and could hardly be detected after 72h. Western blot detection showed that the survivin pro-tein expression rate was 75.2%(12h), 54.3%(24h), 37.5%(48h) and 20.3%(72h), respectively. Thecleaved active subunits of caspase-3 were observed within 24h to 72h after As2O3 treatment. Conclu-Sion :As2O3 induced NCI-H157 cell apoptosis accompanied with survivin downregulation and activationof caspase-3.

     

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