Abstract: Objective : Detection of CK19 mRNA and LUNX mRNA in bone marrow of non - small cell lung cancer (NSCLC) patients in order to discuss the possibility of indicating lung cancer micrometastases by them. Methods : Fluorescence quantitative reverse transcription-polymerase chain reaction (FQ-RT-PCR) and ordinary RT- PCR was used to detect CK19 mRNA and LUNX mRNA in cancer tissue and bone marrow from 62 patients with NSCLC. Lung tissue and bone marrow from 10 patients with benign pulmonary diseases were served as controls. Results : CK19 mRNA and LUNX mRNA was expressed in all the lung tissues, either malignant or benign. In bone marrow, the positive rate of CK19 mRNA for NSCLC was 45.2% (28/62) vs 10%(1/10) in benign controls(P=0.037), the positive rate of LUNX mRNA for NSCLC was 38.7%(24/62) vs 0%(0/10) in benign controls (P=0.017). The positive rate of CK19 mRNA for NSCLC in bone marrow increases according to the stage of disease and nearly reaches a statistical significance (P=0.06), the positive rate of LUNX mRNA for NSCLC in bone marrow increases according to the stage of disease and there is a statistical significance (P=0.02), and for the positive expression in bone marrow of NSCLC there is a relationship between CK19 mRNA and LUNX mRNA (P<0.001). Conclusion : CK19 mRNA and LUNX mRNA are efficient indicating factors on sensitivity and specificity to detect early haematogenous dissemination of cancer cells for patients with NSCLC. This method may lead to an earlier diagnosis of metastasis for lung cancer and do some help to evaluate the cancer more correctly and make the best treatment plan.