Abstract: Objective :To probe values of the EB virus(EBV),mutant p53,bcl-2,c-myc and Rb proteins in onset and development non-Hodgkin's lymphomas(NHL). Methods :EB virus,mutant p53,bcl-2,c-myc and Rb proteins in samples from 140 cases with NHL were detected using immunohistochemistry(S-P method).Meanwhile,samples from 51 cases with reactive lymphatitis were used as control. Results :Compared to the control group,the EBV,mutant p53,bcl-2,c-myc were up-regulated and Rb was down-regulated(P<0.05)in NHL group.EBV was irrelevant to mutant p53,bcl-2 c-myc and Rb in development of the NHL(P<0.05).Both the mutant p53 and bcl-2 were significantly differently expressed in different grade-NHL(P<0.05).EBV,mutant p53,bcl-2 c-myc and Rb were significantly differently expressed in different phase-NHL(P<0.05). Conclusion :There is a positivecor relation between the EBV,mutant p53,bcl-2,c-myc and the on set of the NHL but a negative in Rb;EBV is a factor separating from mutant p53,bcl-2,c-myc,Rb in NHL's development;There was a positive correlation between the mutant p53,bcl-2 and NHL's grade;the EBV,mutant p53,bcl-2 and c-myc were positively correlated to the development of NHL while Rb is a negative one.