Abstract: With the development of clinical diagnostic techniques, increasing numbers of thyroidnodules are detectable. The evaluation of thyroid nodules is important for clinical management. Fine-needle aspiration (FNA) has become the primary diagnostic tool, a technique that has been shown to besafe, fast and accurate. The accuracy rate of detection with FNA can be improved by simultaneousapplication of ultrasound. In addition, specific molecular markers have been discovered in the past tenyears, and they are capable of distinguishing malignant thyroid nodules from benign ones. This articlefocuses on the prospect of FNA combined with molecular markers in the diagnosis of thyroid nodules.