Abstract: Objective: To analyze serum levels of antibodies against EBV EBNA1 and EBZta in individuals in a NPC clan who have non-nasopharyngeal carcinoma (NPC) and to evaluate their risk of getting nasopharyngeal carcinoma. Methods: Serum samples of 41 NPC patients (NPC group), 43 individuals with non-NPC in the NPC clan(non-NPC group) and 90 normal people(normal group) were collected. ELISA was used to detect the EB EBZta IgG and EBNA1 IgA levels. Results: There was no significant difference(t=1.085, t=0.014, P>0.05) in serum EBZta IgG and EBNA1 IgA level between the non-NPC group and the normal group. Compared with the normal group, there was a higher ratio of EBZta IgG positive cases in the non-NPC group (χ²=11.34, P<0.01), while no significant difference was found in the ratio of EBNA1 IgA positive cases between the two groups (χ²=1.304, P>0.05). Conclusion: EBV reactivation is enhanced in individuals in the NPC clan, supporting the fact that NPC clans have a higher risk of getting NPC than normal people.