Abstract: To observe the application of the united detection of tumor markers in the diagnosis and prognosis of  colon cancer. Methods: Up to 102 patients with colon cancer from 2007 to 2010 in  kanghua hospital were enrolled and 50 healthy donors were used as the control. The levels of CEA, CA125, CA19-9, and CA724 were detected whereas their relationship with the clinical stages and the degrees of differentiation of rectal and colorectal carcinoma was analyzed. The effects of the united detection on the susceptibility  and specificity were observed. Results: The levels of CEA, CA125, CA19-9, and CA724 in patients with colon cancer were significantly higher than that in the control group. The levels of the tumor markers were also correlated with the clinical stages and degrees of differentiation. The united detection of tumor markers could significantly improve the susceptibility and specificity. Conclusion: The united detection of serum tumor markers could be conducted to diagnose and prognosticate patients with colon cancer.