Abstract: Kallikrein-related peptidase 14 (KLK14) is a member of the Kallikrein-related peptidases (KLKs) family, a gene which widely exists in various tissues and biological fluids and plays an important physiological role. Current studies indicate that KLK14 can be used as a biomarker for the diagnosis and prognosis of multiple malignant tumors. A secretory serine protease, KLK14 can promote tumor cell metastasis through a variety of ways, and it is involved in the regulation of multiple biological functions such as proliferation, invasion, and migration of malignant tumor cells. Tumor metastasis is the main cause of failure of clinical treatment and death. Therefore, this paper discusses the role and possible mechanism of KLK14 in tumor metastasis based on a review of the existing literature, in order to provide new strategies and ideas for the diagnosis and accurate treatment of malignant tumors.