双调蛋白和白细胞介素10在宫颈癌中的表达及其预后价值

Expression and prognostic value of amphiregulin and interleukin-10 in cervical cancer

    摘要
  • 摘要:
    目的 探讨双调蛋白（amphiregulin，AREG）和白细胞介素10（interleukin-10，IL-10）在宫颈癌（cervical cancer，CC）患者中的表达及与患者预后的关系。
    方法 收集2017年1月至2018年12月收治于新疆医科大学第一附属医院的40例CC患者的宫颈组织作为宫颈癌组，同时选取了30例子宫肌瘤经筛查为阴性的患者的宫颈组织为对照组，采用免疫组织化学染色（immunohistochemistry，IHC）比较两组REG和IL-10的表达；另外收集100例患者（实验组）及60例正常健康个体的血清样本（对照组），使用酶联免疫吸附实验（enzyme-linked immunosorbent assay，ELISA）分别检测两组样本中AREG和IL-10的表达情况，并通过皮尔森相关性分析（Pearson correlation analysis）分析AREG和IL-10的相关性。根据提供样本血清的100例患者分为生存组（80例）和死亡组（20例），绘制Kaplan-Meier曲线分析AREG和IL-10表达与宫颈癌患者生存率的关系；比较两组数据并进行二元Logistic回归和受试者工作特征曲线（ROC）的绘制。
    结果 IHC结果显示组织中AREG和IL-10呈高表达（P<0.05）；ELISA结果显示患者血清中的AREG和IL-10表达增高（P<0.000 1）。Pearson相关性分析显示AREG与IL-10呈正相关（P<0.05，r=0.593 6）；二元Logistic回归分析显示AREG、IL-10和淋巴结转移是患者生存结局的独立危险因素（均P<0.05）；ROC曲线分析显示AREG和IL-10预测患者生存结局的曲线下面积（AUC）分别为0.897和0.874。
    结论 宫颈癌患者组织和血清中AREG和IL-10表达水平升高提示其与的发生发展密切相关，并在宫颈癌患者预后评估中具有较高的临床应用价值。

     

    Abstract:
    Objective To evaluate the expression of amphiregulin (AREG) and interleukin-10 (IL-10) in tissues and peripheral blood from patients with cervical cancer (CC) and to explore their relationship with patient prognosis.
    Methods Cervical cancer tissues of 40 cervical cancer patients admitted to The First Affiliated Hospital of Xinjiang Medical University from Jan 2017 to Dec 2018 were collected as the cervical cancer group, and the cervical tissues of 30 patients with negative screening results of uterine fibroids were selected as the control group.Immunohistochemistry (IHC) was performed to compare AREG and IL-10 expression between the two groups. Serum samples from 100 patients with CC (cervical cancer group) and 60 normal healthy individuals (control group) admitted during the same period were analyzed for AREG and IL-10 expression using enzyme-linked immunosorbent assay (ELISA). Pearson correlation analysis was used to evaluate the relationship between AREG and IL-10. The 100 patients with cervical cancer were assigned into survivor (80 patients) and deceased (20 patients) groups. A Kaplan–Meier plot was used to analyze the relationship between AREG and IL-10 expression and the survival rates among patients with CC. Binary Logistic regression and receiver operating characteristic curve (ROC) analyses were performed to evaluate prognostic factors.
    Results IHC results indicated that AREG and IL-10 were highly expressed in CC tissues compared to normal tissues (P<0.05). ELISA results revealed significantly elevated serum levels of AREG and IL-10 in cancer patients (P<0.000 1). Pearson correlation analysis showed that AREG correlated positively with IL-10 (P<0.05, r=0.593 6). Binary Logistic regression analysis identified AREG, IL-10, and lymph node metastasis as independent risk factors impacting survival of patients with CC (all P<0.05). ROC curve analysis showed AREG and IL-10 had high predictive value for survival (AUC=0.897 and 0.874, respectively).
    Conclusions Increased AREG and IL-10 expression in tissues and serum of patients with CC suggest that AREG and IL-10 are closely related with the occurrence and progression of the disease. These biomarkers hold significant clinical value for predicting the prognosis of patients with CC.

     

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