Abstract:
Objective To evaluate the accuracy and feasibility of an automated scanning and uptake system to assist pathologists with human epidermal growth factor receptor 2 (HER2) FISH interpretation.
Methods HER2 gene amplification is detected using FISH, and “result interpretation by independent pathologists” is regarded as the “gold standard.” The consistency of “human-machine dialogue results” (use of a CytoVision* system combined with manual interpretation) and “CytoVision*-based automated interpretation” with the “gold standard” was assessed.
Results Consistency between "human-machine dialogue results" and the "gold standard" can surpass 91%, with the former method saving up to 50% of the manual operation time. The tendency of each cell nucleus's HER2 copy number to be "underestimated" is the main reason for the low sensitivity observed in cases with low copy number amplification and HER2 heterogeneous expression cases in "human-machine dialogue interpretation."
Conclusions Automatic FISH image analysis and uptake systems simulate the process of manually interpreted cell selection, ensure random cell selection, and improve work efficiency. With its accurate selection of the hybridization region and “human-computer dialogue,” the system is expected to “replace” interpretation by independent pathologists.
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