Abstract: Objective:To investigate the expression of heparanase (HPSE) and matrix metalloproteinase-9 (MMP- 9) in pancreatic carcinoma, chronic pancreatitis, normal pancreatic tissue and the correlation between the two, and to analyze the relationship among the proteins, the infiltration, and metastasis of pancreatic carcinoma. Methods:Immunohistochemi -cal staining (SP method) was used to measure the HPSE and MMP- 9 expressions in 92cases with pancreatic carcinoma, 24with chronic pancreatitis (CP) and10with normal pancreatic tissue samples. The correlation between the expression of HPSE and MMP-9 and the clinico-pathologic index was analyzed and Pearson rank correlation analysis was set up for sta -tistical treatment. Results: The expression of HPSE was significantly higher in pancreatic carcinoma than in chronic pancre -atitis and normal pancreatic tissue ( P<0.05). The positive expression of HPSE protein had a positive correlation with the in -filtration of peripheral tissue and lymph node metastasis ( P<0.05), and had a negative correlation with the gender, age and histological grading (P>0.005 ). The expression of MMP-9 was significantly higher in the pancreatic carcinoma than in the chronic pancreatitis and normal pancreas (P<0.05). The positive expression of MMP- 9 protein positively correlated with the infiltration of peripheral tissue and the distant metastasis (P<0.05), and correlated negatively with the sex, age, and tumor differentiation of patients ( P>0.005 ). Both HPSE and MMP- 9 had a positive co-expression rate in pancreatic carcinoma. The expressions of HPSE correlated positively with that of MMP-9 in pancreatic carcinomas ( P<0.05). The co-expression rate related to the tumor infiltration of other peripheral organs and nodal metastases ( P<0.05). Multivariate analysis showed that neither HPSE nor MMP- 9 were independent prognostic factors. Conclusion:HPSE and MMP-9 expression in pancreat-ic carcinoma may be associated with the infiltration and metastasis of carcinoma. These should be regarded as the new markers of pancreatic carcinoma for combined detection, with important clinical significance. MMP-9 may also up-regulate the level of HPSE expression, and facilitate the HPSE-mediated tumor infiltration and metastasis.