Abstract: Objective: To construct a TXR1 expression vector and study the expression of the protein in a human breast cancer cell line. Methods:TXR1 cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and was then inserted into pEGFP-C3 vector to construct pEGFP-TXR1. After confirmation by DNA sequencing, the recom -binant pEGFP-TXR 1 was transfected into cultured MCF- 7 cells by lipofectamine 2000. The expression of TXR 1 in trans -fected cells was assessed by RT-PCR and immunoblotting, and MTT assay was used to detect cell proliferation and con -struct an inhibition curve for Taxol. Results: TXR1 expression was highly expressed in MCF- 7 cells containing the recombi-nant expression vector. The survival rate of MCF-7 cells with high expression of TXR1 increased. Conclusion:A recombi-nant TXR1 expression vector has been successfully constructed. It expresses TXR 1 in MCF- 7 breast cancer cells and cells with a high expression level of TXR1 acquire Taxol resistance.