Abstract: Objective: To investigate the dynamic changes in the expression of focal adhesion kinase ( FAK ), matrix metallopro-teinase-2 ( MMP- 2 ), and matrix metalloproteinase 9 ( MMP- 9 in Fadu cells after stimulation with different concentrations of plate-let-derived growth factor-BB (PDGF-BB ) and to explore their effect on cell proliferation and invasion. Methods:After Fadu cells are stimulated with different PDGF-BB concentrations, the dynamic changes in FAK, MMP-9, and MMP- 2 mRNA expression in the cells were determined by reverse transcriptase polymerase chain reaction, and the changes in cell proliferation and invasive ability were de-termined with an MTT assay and a Transwell system assay, respectively. Western blot analysis was used for the protein expression. Re-sults: The expression of FAK, MMP- 9, and MMP-2 mRNA in Fadu cells was upregulated after stimulation with PDGF-BB and they reached their peak under 20or 10ng/mL of PDGF-BB ( P < 0.05). FAK, MMP-9, and MMP-2 protein expression had the similar changes and all reached their peak in the 20ng/mL group. Cell proliferation was not changed after induction. The number of invasive cells increased and reached their peaks in the20ng/mL group ( 20.12± 4.5 ), which were significantly different from that in the control group ( P < 0.05). Conclusion:PDGF-BB upregulates the expression of FAK, MMP- 9, and MMP- 2, which facilitates the invasion of Fadu cells, but PDGF-BB has no effect on their proliferation.