Abstract: To investigate the expression of CXCL1 and its receptor CXCR2 in human gastric carcinoma tissues as well as to further explore its effect and clinical significance in the occurrence, infiltration, and metastasis of gastric carcinoma. Methods: The expression patterns of CXCL1 and CXCR2 in the normal and tumor tissues of 40 patients with gastric cancer were determined by immunohistochemical staining and Western blot analysis. Statistical analysis of various expression patterns and clinicopathologic features was conducted, and reverse transcription-polymerase chain reaction was used to detect the CXCL1 mRNA expression in 19 subjects. Results: The expression of CXCL1 and CXCR2 at protein and mRNA levels were both high in the gastric carcinoma and metastasized lymph nodes, which were correlated with the clinical stages ( P < 0.05 ). Conclusion: The high expression of CXCL1 and CXCR2 is related to the occurrence, development, and metastasis of gastric carcinoma and plays an important role in the lymphatic metastasis of the cancer.