Abstract: To investigate the expression of the receptor of advanced glycosylation end-products ( RAGE ) in human cervical squamous cell carcinoma ( CSCC ) and to explore the relationship of this expression to several factors such as differentiation degree, size and stage of CSCC, and invasion, as well as metastasis of CSCC. Methods: Immunohistochemical staining of tissue microarrays was conducted to detect the expression of RAGE in the following tissue samples: 30 carcinomas in situ, 90 invasive CSCCs without metastasis, 30 invasive CSCCs with metastasis, and 30 cases with normal cervical squamous epithelia. Western blot analysis was used to detect the expression of RAGE in CSCC. Results: The positive expression rates of RAGE were 48.0% in CSCC ( 72/150 ) and 13.3% in normal squamous epithelial tissues of the cervix ( 20/150 ), indicating significant differences between the two conditions. The overexpression of RAGE was correlated with tumor progression, stage, invasion, and metastasis of CSCC ( P < 0.01 ), but it had no relation with the degree of differentiation and tumor size ( P > 0.05 ). Conclusion: Overexpression of RAGE in CSCC, especially in cervical squamous epithelial tissues with tumor metastasis, can be used as one of the parameters for determining tumor invasion, metastasis, and prognosis.