Abstract: To investigate TGFBI promoter methylation and its possible correlation with the expression of CyclinD1 protein in epithelial ovarian carcinoma tissues and evaluate the effect of TGFBI promoter methylation on the development of epithelial ovarian carcinoma. Methods: TGFBI promoter methylation was detected by methylation-specific polymerase chain reaction in 20 cases of normal epithelial ovarian tissues ( Group A ), 20 cases of benign epithelial ovarian tissues ( Group B ), and 30 cases of epithelial ovarian carcinoma tissues ( Group C ). The expression of CyclinD1 protein was detected by immunohistochemistry. Results: The rate of TGFBI methylation in epithelial ovarian carcinoma tissues was higher than in benign and normal epithelial ovarian tissues ( P < 0.05 ). The expression of CyclinD1 protein in epithelial ovarian tissues followed the trend Group C > Group B > Group A ( P < 0.0167 ). The high expression of CyclinD1 protein was closely correlated with TGFBI promoter methylation ( r = 0.505. P < 0.05 ). Conclusion: TGFBI gene promoter methylation inactivates the TGFBI gene, causing increased CyclinD1 protein expression, which stimulates abnormal cellular proliferation. This mechanism may be involved in the development of epithelial ovarian carcinoma.