检测外周血Lunx mRNA表达在诊断非小细胞肺癌微转移中的临床价值

Clinical Significance of Lunx mRNA Detection in the Peripheral Blood of Non-Small Cell Lung Cancer Patients

    摘要
  • 摘要:
      目的  探讨检测非小细胞肺癌(NSCLC)患者外周血Lunx mRNA表达对于诊断肿瘤微转移的临床价值。
      方法  采用荧光定量RT-PCR技术分别对98例NSCLC患者和20例正常对照组外周血Lunx mRNA的表达进行检测分析。
      结果  NSCLC组患者外周血Lunx mRNA的阳性率为74.5%, 显著高于对照组的15%(χ2=25.642, P < 0.05);Ⅲ期NSCLC患者的阳性率显著高于Ⅰ期和Ⅱ期患者的阳性率(χ2=21.014, P < 0.05);淋巴结转移阳性和阴性患者的外周血中CK19mRNA阳性率分别为93.2%和46.2%, 差异有统计学意义(χ2=27.372, P < 0.05);不同细胞分化程度与NSCLC患者外周血微转移阳性率有密切关系(χ2=30.004, P < 0.05);NSCLC患者外周血微转移与年龄、性别、吸烟史、原发肿瘤部位无密切关系(P>0.05)。
      结论  Lunx mRNA的高表达可能与非小细胞肺癌的微转移有关, 可作为检测NSCLC患者微转移的分子标志物, 其有助于早期诊断肺癌转移, 指导临床分期和治疗。

     

    Abstract:
      Objective  To evaluate the clinical significance of Lunx mRNA detection in the peripheral blood of patients with non-small cell lung cancer (NSCLC).
      Methods  Lunx mRNA expression was detected by fluorescence quantitative reverse transcription polymerase chain reaction in the peripheral blood from 98 NSCLC patients and a control group of 20 healthy adults.
      Results  The positive rate of Lunx mRNA in peripheral blood in the NSCLC group was 74.5%, significantly higher than that in the control group (χ2 = 25.642, P < 0.05). The expression rate increased significantly as the clinical stage progressed (χ2 = 21.014, P < 0.05). Significant difference was found among the different lymph node metastasis status ( χ2 = 27.372, P < 0.05) and different cell differentiation status (χ2 = 30.004, P < 0.05). No significant difference was noted between the positive rates of Lunx mRNA for different ages, gender, history of smoking, and primary tumor position of NSCLC (P > 0.05).
      Conclusion  Higher Lunx mRNA expression in the peripheral blood of NSCLC patients may be related to the micro-metastasis of the disease and might be a valuable marker for micrometastasis. Lunx mRNA detection is significant for the early prediction of NSCLC prognosis and metastasis.

     

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