蛇毒精氨酸酯酶Agkihpin抑制人肝癌SMMC-7721细胞株MRP1表达

The Inhibition of Venin Arginine Esterase Agkihpin in the Expression of MRP1 in the SMMC-7721 Cell Strain

    摘要
  • 摘要:
      目的   探讨蛇毒精氨酸酯酶Agkihpin对人肝癌细胞株SMMC-7721中多药耐药相关蛋白1(multidrug resistance associated protein 1, MRP1)表达的影响, 并阐明Agkihpin抑制人肝癌SMMC-7721细胞活力的机制。
      方法   目的: 方法: 采用不同浓度的Agkihpin处理SMMC-7721细胞72 h后, 应用免疫细胞化学、Western blot和RT-PCR等方法检测MRP1在SMMC-7721细胞中的转录和表达。
      结果   不同浓度Agkihpin作用SMMC-7721细胞72 h后MRP1表达均降低, 显示Agkihpin可显著下调SMMC-7721细胞中MRP1转录和表达(P < 0.05), 并呈现出一定的浓度依赖效应。
      结论   Agkibpin能抑制人低分化肝癌细胞株SMMC-7721中MRP1的表达, 并呈一定程度的浓度依赖效应, 提示Agkihpin在一定程度上可用于提高肝癌细胞对化疗药物的敏感性。

     

    Abstract:
      Objective   To explore the effects of Agkihpin on the expression of multidrug resistance-associated protein 1(MRP1) in the SMMC-7721 cell line and to describe the mechanism of SMMC-7721 inhibition by Agkihpin.
      Methods   The cultured cells were treated with different concentrations of Agkihpin for 72 h.The transcription and expression levels of MRP 1 in SMMC-7721 were assayed using immunocytochemistry, Western blot, and reverse transcription-polymerase chain reaction.
      Results   Compared with the control group, both the transcription and the expression levels of MRP1 in SMMC-7721 were significantly reduced after treatment with different concentrations of Agkihpin(P < 0.05) in a concentration-dependent manner.
      Conclusion   Agkihpin can inhibit the transcription and expression of MRP1 in SMMC-7721 cells, which is likely responsible for the inhibition of cellular vitality and, to some extent, the enhancement of the sensitivity of the hepatocellular carcinoma cells to chemotherapeutics.

     

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