广西宫颈癌患者HPV感染情况的分子流行病学调查

Molecular Epidemiology Investigation on Guangxi Cervical Cancer Patients with Human Papillomavirus Infection

    摘要
  • 摘要:
      目的  调查人乳头瘤状病毒（Human papillomavirus，HPV）各亚型在广西壮族自治区宫颈癌患者中的分布情况，为宫颈癌疫苗的研制提供理论依据。
      方法  应用凯普HPV核酸扩增分型检测试剂盒对515例广西宫颈癌患者的宫颈组织DNA进行21种HPV亚型的检测；并应用实时荧光定量PCR对HPV16、18和58型阳性样本各50、48和41例的病毒负载量进行了绝对定量检测。
      结果  1）检测的515例样本中473例HPV阳性，HPV总阳性率为91.84%。HPV16的阳性率最高，为81.40%；其次为HPV18（10.15%）；第三位是HPV58（8.67%）。排在前五位的HPV亚型依次为HPV 16、18、58、52及31。2）检测样本中HPV16、18和58型的病毒负载量几何均数分别为2.398 5、0.017 3、0.038 1。HPV16分别与HPV18和58型的病毒负载量比较，有显著性差异（P < 0.05）；HPV18与58型的病毒负载量比较，无显著性差异（P>0.05）。
      结论  广西宫颈癌患者中有较高的HPV阳性率，其中HPV16型排在首位，HPV18和58虽然分列第二、三位，但阳性率明显低于HPV16。HPV16的病毒负载量远远高于HPV18和58，说明HPV16的高病毒负载量与其高阳性率可能存在一定关系。

     

    Abstract:
      Objective  This study aims to investigate the distribution of human papillomavirus (HPV) subtypes in Guangxi cervical cancer patients and to provide theoretical bases for research on Guangxi HPV vaccine.
      Methods  A total of 21 subtypes of HPV from 515 cervical cancer cases in the Guangxi Zhuang Autonomous region were detected using flow-through hybridization and gene chip technology.The viral loads of HPV16, HPV18, and HPV58 from 50, 48, and 41 patients, respectively, were examined by quantitative fluorescence PCR.
      Results  1) Among the 515 cervical cancer cases, 473 (91.84%) were HPV positive. The positive rate of HPV16 was the highest (81.40%), followed by HPV18 (10.15%) and then HPV58 (8.67%). The top five HPV subtypes in Guangxi cervical cancer patients were HPV16, HPV18, HPV58, HPV52, and HPV31. 2) The viral load geometric means of HPV16, HPV18, and HPV58 were 2.3985, 0.0173, and 0.0381, respectively. The viral load geometric mean of HPV16 was significantly higher than that of HPV18 and HPV58 (P < 0.05). However, no significant difference in the viral load geometric mean was found between HPV18 and HPV58 (P>0.05).
      Conclusion  The positive rate of HPV is very high in the Guangxi region. The positive rate of HPV16 is the highest, followed by HPVl8 and then HPV58. The positive rate and average viral load of HPV16 were both higher than those of HPV18 and HPV58. Thus, a relationship between the high viral load and high positive rate of HPV may exist.

     

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