miRNA-375对胰腺癌细胞Panc-1增殖和凋亡的影响

宋世铎; 周健; 何宋兵; 朱东明; 赵华; 张子祥; 王毅; 李德春

doi:10.3969/j.issn.1000-8179.2013.01.006

miRNA-375对胰腺癌细胞Panc-1增殖和凋亡的影响

Effects of miRNA-375 on cell proliferation and apoptosis in the pancreatic cancer cell line Panc-1

摘要

摘要:
目的探讨miRNA-375的外源性表达对人胰腺癌细胞Panc-1增殖和凋亡的影响。
方法将miRNA-375表达质粒或对照质粒转染Panc-1细胞, qRT-PCR方法检测miRNA-375在转染细胞中的表达水平, 细胞计数法、流式细胞术分别检测外源性miRNA-375表达后Panc-1细胞增殖、凋亡和周期的变化情况。
结果 miRNA-375表达质粒组与对照组比较, 其miRNA-375表达诱导性升高, 细胞增殖受到抑制, 细胞凋亡率增加, 细胞周期阻滞于G₀~G₁期。
结论在胰腺癌细胞Panc-1中增加miRNA-375的表达, 可抑制细胞增殖, 促进细胞凋亡和细胞周期阻滞, miRNA-375在胰腺癌细胞Panc-1中可能发挥潜在的抑癌基因作用。

Abstract:
Objective This study aimed to investigate the effect of the ectogenesis expression of miRNA-375 on cell proliferation and apoptosis in the human pancreatic cancer cell line Panc-1.
Methods After transfection of the miRNA-375 vector, the expression levels of miRNA-375 in the Panc-1 cells were determined by quantitative real-time polymerase chain reaction.Cell counting was performed to evaluate the effects of miRNA-375 expression on cell proliferation.Flow cytometry was used to analyze apoptosis and the cell cycle.
Results The expression levels of miRNA-375 were highly induced after miRNA-375 transfection.miRNA-375 restoration also inhibited cell proliferation, promoted cell apoptosis, and induced cell cycle arrest at G₀-G₁.
Conclusion The abnormal expression of miRNA-375 in the pancreatic cancer cell line Panc-1 inhibits the canceration of cells by interfering with cellular biological behaviors, such as proliferation and apoptosis.miRNA-375 may act as a potential tumor suppressor in the pancreatic cancer cell Panc-1.

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