EHD2负向调控前列腺癌细胞的增殖与转化能力

Effect of EHD2 on proliferation and transformation of prostate cancer cells

    摘要
  • 摘要:
      目的  研究EHD2在前列腺癌中的表达以及EHD2表达水平对前列腺癌细胞系增殖与转化能力的影响。
      方法  免疫组织化学方法检测EHD2在14例前列腺癌组织及7例前列腺增生组织中的表达。用EHD2干扰表达质粒、过表达质粒和对照载体分别转染293T细胞, 制备慢病毒, 感染DU145细胞, 建立稳定细胞系。用Western blot技术检测EHD2表达情况。采用细胞计数法、二维克隆形成实验、Soft Agar实验分别检测EHD2沉默或过表达对前列腺癌细胞增殖能力、克隆形成能力和转化特性的影响。
      结果  免疫组织化学显示EHD2在前列腺癌组织中表达量明显低于前列腺增生组织。EHD2干扰表达后DU145细胞的增殖能力、二维克隆形成能力、三维克隆形成能力均明显增强(P < 0.01);EHD2过表达后DU145细胞的增殖能力和三维克隆形成能力均减弱(P < 0.01和P < 0.05)。
      结论  EHD2的表达水平对DU145前列腺癌细胞的增殖与转化特性具有明显的调节作用, EHD2可能是前列腺癌的新抑癌基因。

     

    Abstract:
      Objectives  This research examined EHD2 expression level in prostate cancer and identified its effects on proliferation and transformation of prostate cancer cells.
      Methods  Immunohistochemistry was used to detect EHD2 expression level in prostate cancer tissue and benign prostatic hyperplasia.The lentiviral system facilitates EHD2 overexpression or silencing in DU145 cells.Cell counting, colony formation, and soft agar experiments were used to determine cell proliferation and transformation characteristics.
      Results  EHD2 expression decreased in cancer cells compared with cells in hyperplasia tissue.EHD2 downregulation promoted DU145 cell growth and colony formation(P < 0.01).Opposite effects were observed with EHD2 overexpression.
      Conclusion  EHD2 expression was suppressed in prostate tumor cells.Therefore, EHD2 may be a novel tumor suppressor in prostate cancer.

     

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