Abstract:
Objective This study aimed to explore miR126 expression and its relationship with vascular endothelial growth factor(VEGF) in esophageal squamous cell carcinoma, as well as the effect of miR126 expression on an esophageal carcinoma cell line.
Methods Four patients with esophageal squamous cell carcinoma were randomly selected in our hospital from 2011.3 to 2011.4.Tumor and normal tumor-adjacent tissue specimens were collected from the patients, and miRNA was extracted.The expression of miR126 was detected by real-time quantitative PCR.Furthermore, total mRNA and protein were extracted to detect VEGF expression by real-time quantitative PCR and Western blot analysis.miR126-like mRNA was then transfected to the TE-1 cell line to determine its effect on the proliferation of TE-1 cells and VEGF expression.
Results miR126 expression was significantly downregulated in esophageal carcinoma tissues compared with normal esophageal tissues.By contrast, the expression of VEGF RNA and protein significantly increased in esophageal carcinoma tissues compared with normal esophageal tissues.The incorporation of BrdU in cells transfected with miR126-like mRNA significantly increased compared with untransfected cells, whereas the levels of VEGF RNA and protein significantly decreased in transfected cells.
Conclusion miR126 expression was downregulated and VEGF was upregulated in esophageal squamous cell carcinoma.Therefore, miR126 may downregulate the expression of VEGF and inhibit the proliferation of TE-1 cells.
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