二氢青蒿素对胃癌细胞血管生成及相关基因表达的影响

Influence and significance of DHA on expression of angiogenesis-related genes in SGC7901 cells

    摘要
  • 摘要:
      目的  检测二氢青蒿素（DHA）对胃癌SGC7901细胞中血管生成及相关基因表达的影响及意义。
      方法  不同浓度（5、10、20、40、80 μmol/L）DHA作用SGC7901细胞24、48和72 h后，通过MTT法检测DHA对SGC7901细胞增殖的抑制作用。利用荧光定量RT-PCR方法检测细胞中人表皮生长因子（VEGF-C）、环氧合酶-2（COX-2）、血管细胞黏附分子-1（VCAM-1）和PTEN的mRNA的表达量；利用Western blot方法检测其蛋白表达量。
      结果  SGC7901细胞经过不同浓度DHA处理24 h以及50 μmol/L DHA处理24、48和72 h后，SGC7901细胞增殖受到明显抑制，细胞中VEGF-C、COX-2、VCAM-1和PTEN的mRNA及蛋白的表达水平均都受到抑制；PTEN的mRNA及蛋白的表达水平则呈升高趋势。
      结论  DHA通过减少胃癌SGC7901细胞中VEGF-C、COX-2和VCAM-1基因的表达，促进PTEN的表达，抑制了肿瘤细胞的生长。

     

    Abstract:
      Objective  To investigate the influence and significance of DHA on expression of angiogenesis-related genes in SGC7901 cells.
      Methods  SGC7901 were treated with DHA (5, 10, 20, 40, and 80 μmol/l) for different times (24, 48, and 72 h), and the growth inhibition was detected by MTT. The expression of vascular endothelial growth factor (VEGF-C), cyclooxygenase-2 (COX-2) vascular cell adhesion molecule-1 (VCAM-1), and PTEN mRNA were detected by fluorescence-based quantitative polymerase chain reaction (qRT-PCR). Their corresponding protein levels were tested by Western blot.
      Results  DHA significantly inhibited the growth of SGC7901 cells in a dose- and time-dependent manner (P < 0.05). The expression of the angiogenesis-related genes significantly changed, as shown by RT-PCR and Western blot analyses. Compared with the control group, the expressions of VEGF-C, COX-2, and VCAM-1 were down-regulated, whereas the expressions of PTEN were up-regulated, after DHA treatment (P < 0.05).
      Conclusion  DHA inhibits cell growth in gastric cancer SGC7901 cells. The effect may be due to its reduction of VEGF-C, COX-2, and VCAM-1 gene expression, as well as its promotion of PTEN expression in gastric cancer cells

     

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