黄芩素对人乳腺癌MDA-MB-231细胞株SATB1表达的影响

Effect of Baicalein on the expression of SATB1 in MDA-MB-231 cells

    摘要
  • 摘要:
      目的  观察黄芩素对人乳腺癌MDA-MB-231细胞SATB1蛋白表达的影响。
      方法  用MTT法、划痕愈合实验观察不同浓度黄芩素干预后 MDA-MB-231 细胞增殖和运动迁移能力的变化；用 Western Blot 法检测黄芩素干预后 MDA-MB-231 细胞SATB1蛋白表达的变化。
      结果  随作用时间的延长和药物浓度的增加，黄芩素对MDA-MB-231细胞增殖和运动迁移的抑制作用逐渐增强，呈明显的时间-剂量依赖性（P＜0.05）；黄芩素可显著降低MDA-MB-231细胞中SATB1蛋白的表达，而且随着药物浓度增加，SATB1蛋白表达量逐渐减少（P＜0.05）。
      结论  黄芩素可通过抑制SATB1的表达抑制肿瘤细胞的恶性增殖、侵袭和迁移能力。

     

    Abstract:
      Objective  To investigate the effect of Baicalein on SATB1 expression and its relation to proliferation, invasivenessand migration of MDA-MB-231 cells.
      Methods  Colorimetric 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT)and wound healing assays were used to detect the cell proliferation and the migration changes of MDA-MB-231 cells when treated withdifferent concentrations of Baicalein. SATB1 expression in MDA-MB-231 cells was detected by Western blot.
      Results  Along with thereaction time and increase drug concentration, the inhibitory effect of Baicalein on proliferation and migration of MDA-MB-231 cellsgradually increased in a time- and dose- dependent manner (P＜0.05). After treatment with Baicalein for 48 h, an obvious decrease wasobserved in the level of SATB1 protein expression of MDA-MB-231 cells in a dose-dependent manner (P＜0.05).
      Conclusion  Baicalein can suppress MDA-MB-231 cells growth and migration by inhibiting its proliferation in a time- and dose- dependent manner. It canalso down-regulate the expression of SATB1.

     

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