Abstract: Objective:The diagnostic sensitivity of alpha-fetoprotein (AFP) in patients with primary liver cancer is poor and un -satisfactory in the clinical diagnosis of patients with primary liver cancer. Recent studies have demonstrated the upregulation of serum primary hepatic carcinoma (PHC) and Golgi glycoprotein73(GP 73) in patients with PHC. This study aims to discuss the clinical signif-icance of GP73and AFP in the serum on the diagnosis of primary liver cancer. Methods:The serum concentrations of GP73and AFP were retrospectively analyzed in46hepatocellular carcinoma patients, 30cases of patients with liver cirrhosis alone, 44patients with other digestive tumors, and40healthy subjects as controls. Quantitative euzymelinked immunosorbent assay and electrochemilumines -cence immunoassay were conducted to determine the GP73and AFP levels in the serum, respectively.Results: The sensitivity of PHC diagnosis significantly increased with GP 73(73.9%) compared with AFP (56.5%), and the difference was statistically significant (P< 0.01). The sensitivity of AFP combined with GP 73significantly improved to 86.9% compared with the sensitivity of a single indicator (P<0.01). Conclusion:GP73may be used as one of the PHC serum markers for diagnosis. The combined detection of GP 73and AFP offers great potential for future application in the diagnosis of PHC.