miR-30通过调节CD90表达抑制肝细胞肝癌

miR-30 functions as a tumor suppressor by modulating CD90 in hepatocellular carcinoma

    摘要
  • 摘要:
      目的  miRNA能通过转录后调节靶基因的表达量，并在致癌过程中发挥抑癌或促癌作用。研究发现miR-30家族在人类肿瘤中起着重要作用，并参与维持干细胞特性的上皮细胞间质化过程，本研究主要识别miR-30家族与肝细胞肝癌的关系。
      方法  应用qRT-PCR实验方法检测93例肝细胞肝癌患者癌组织和癌旁正常组织中miR-30c，miR-30b和miR-30e表达水平，另对121例肝细胞肝癌患者癌组织进行miR-30家族的表达及CD90免疫组织化学表达检测，分析miR-30家族与CD90在肝细胞肝癌中的相关性。
      结果  本研究发现miR-30c(P < 0.001)、miR-30b(P=0.004)和miR-30e(P < 0.001)与癌旁正常组织相比，癌组织中表达显著下调。CD90蛋白在癌组织中的表达水平显著高于癌旁正常组织(P=0.007)。MiR-30c(P=0.032)和miR-30e(P=0.015)在CD90阴性表达的患者中表达水平显著高于CD90阳性表达的患者。
      结论  miR-30家族在肝细胞肝癌中可作为抑癌miRNA，并通过调节CD90蛋白来发挥这一作用。

     

    Abstract:
      Objective  miRNA can post-transcriptionally regulate the expression of target genes and act as oncogenes or tumor suppressor genes in tumorigenesis. Emerging evidence demonstrates that the miR-30 family may perform an important function in human cancers and can regulate epithelial-mesenchymal transition, which has a critical role in cancer stem cells. This research was conducted to identify the possible association between the miR-30 family and hepatocellular carcinoma (HCC).
      Methods  The expression of miR-30c, miR-30b, and miR-30e in 93 tumor tissues and adjacent tissues were measured by real-time reverse transcription PCR. Furthermore, 121 tumor tissues from an independent cohort were selected to measure the expression level of miR-30 family and CD90 by immunohistochemistry. The relationship between miR-30 family and CD90 protein expression was analyzed.
      Results  The expression levels of miR-30c (P < 0.001), miR-30b (P=0.004), and miR-30e (P < 0.001) were significantly decreased in tumor tissues compared with adjacent tissues, and the expression level of CD90 in tumor tissues was significantly higher than that in adjacent normal tissues (P= 0.007). In addition, the expression levels of miR-30c (P=0.032) and miR-30e (P=0.015) were significantly high in negative staining for CD90 when compared with positive staining for CD90.
      Conclusion  Taken together, we suggest that the miR-30 family may act as a tumor suppressor in HCC development and may modulate CD90 protein expression.

     

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