Abstract: The FOXO gene, which is a member of the Forkhead box (Fox) gene family, is found in drosophila and is widespread in the eukaryotes of many organisms, including yeast and mammals. This gene transcripts the FOXO protein family, which has impor -tant roles in metabolism, cellular proliferation, stress resistance, and apoptosis. Activation of cell survival pathways, such as phos -phoinositide-3-kinase/AKT or RAS/mitogen-activated protein kinase, phosphorylates FOXOs at different sites, thus regulating the nu -clear localization or degradation of FOXOs. A growing number of evidence indicates that FOXO acts as a tumor suppressor in a wide variety of tumors. Scientists have focused on the correlation between tumors and FOXO. In combination with other chemo- or radiother-apy treatments, drugs designed to increase or restore FOXO activity may constitute a potential strategy in cancer treatment. This review summarizes details on the regulation of FOXO and the latest research progress of FOXO as a molecule therapeutic target.