Abstract: Objective:To explore the expression of aquaporin 1 (AQP 1) in human glioma tissues and its relationship with the clini -copathological parameters and prognosis of this tumor. This study also observed the function of AQP1 in the proliferation and invasion of LN229 glioblastoma cells. Methods:The expression of AQP1 in 135 cases of glioma was detected by immunohistochemical meth -od, and the correlation between AQP 1 and pathological features of glioma was analyzed. The relationship of AQP1 with survival was al-so investigated using 103 specimens with complete clinical data. AQP1 was successfully transfected into LN229 cells with lentiviral vector, and the expression of AQP 1 protein was tested by Western blot. Cell proliferation was detected by using methyl thiazolyl tetrazo-lium assay, whereas cell invasion was determined by Transwell assay. Results: The expression of AQP1 was positively correlated with pathological grading. High AQP1 expression was associated with poor prognosis ( P<0.05). Moreover, the overexpression of AQP1 can significantly increase the proliferation and invasion of LN 229 cells (P<0.05). Conclusion:AQP1 is closely associated with the progres -sion of glioma. Upregulation of the AQP 1 expression promoted the proliferation and metastasis of glioma cells. These findings indicat-ed that AQP 1 can function as a therapeutic target for glioma in future research.