Abstract: Objective:To investigate the expression of C-X-C chemokine ligand-14(CXCL14) and epidermal growth factor receptor (EG -FR) in human gastric cancer and to analyze the relationship of CXCL14with clinicopathological features. Methods:The expression of CXCL 14and EGFR was detected by Immunohistochemical SP method in 121 cases of gastric cancer tissue, 62cases of the adjacent non-tumor gastric mucosa, and 60cases of allogeneic non-tumor gastric mucosa. Results: The positive rates of CXCL 14and EGFR expres -sion were 80. 17% and 48. 76% in gastric cancer, respectively, and both were significantly higher in the adjacent non-tumor gastric mu -cosa and allogeneic non-tumor gastric mucosa. The differences were significant (P<0. 01). Overexpression of CXCL 14was closely corre  lated with the depth of cancer invasion, differentiation, and clinical stage, and the differences were significant ( P<0. 05). Overexpres -sion of EGFR was correlated with cancer differentiation, lymph node metastasis, and clinical stage. The differences were significant ( P< 0. 05). Based on the Spearman correlation analysis, the expression of CXCL 14and EGFR in gastric cancer was positively correlated ( rs=0. 195 , P<0. 05). Conclusion: Abnormal CXCL14expression in gastric cancer may be associated with the occurrence and development of gastric cancer. CXCL 14expression is positively correlated with EGFR expression, suggesting that the two have a synergistic effect in gas-tric cancer development.