胃癌中DNA甲基化对miR-200c 141表达影响的研究

MiR-200c/141 methylation inhibits the expression of miR-200c and miR-141 in gastric cancer

    摘要
  • 摘要:
      目的  探讨胃癌组织中miR-200c/141CpG岛的甲基化水平与miR-200c/141表达水平和临床病理特征的相关性。
      方法  运用实时定量PCR（qRT-PCR）和BS-MSP方法检测胃癌组织和癌旁组织中miR-200c/141CpG岛的表达与其基因甲基化水平。统计学分析miR-200c/141CpG岛的甲基化水平与miR-200c/141水平和临床病理特征的关系。
      结果  miR-200c/141CpG岛的甲基化水平在胃癌组织中显著升高，miR-200c和miR-141的水平显著降低，miR-200c/141CpG岛的甲基化水平与miR-200c和miR-141的水平呈负相关。
      结论  胃癌组织中升高的miR-200c/141CpG岛的甲基化水平诱导miR-200c和miR-141表达降低。

     

    Abstract:
      Objective  This work aims to detect the levels of miR-200c/141 methylation and miR-200c/141 in gastric cancer tissue and investigate the relationship between miR-200c/141 expression and clinical parameters.
      Methods  The methylation status of miR-200c/ 141 CpG island and miR-200c/141 in gastric cancer tissue specimens was evaluated by qRT-PCR or BS-MSP method. We analyzed the relationship among the methylation status of miR-200c/141 CpG island, expression level of miR-200c or miR-141, and clinical parameters.
      Results  The status of miR-200c/141 CpG island methylation in gastric cancer tissue was significantly higher compared with that in paracarcinoma tissue. MiR-200c and miR-141 were markedly decreased in gastric cancer tissue compared with those in adjacent tissue. MiR-200c/141 CpG island methylation was negatively related with the expression of miR-200c and miR-141 in gastric cancer specimens.
      Conclusion  The upregulation of miR-200c/141 CpG methylation inhibits miR-200c/141 expression in gastric cancer tissue.

     

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