Erbin在食管鳞状细胞癌中的表达及其与患者预后的关系
Expression of Erbin in esophageal squamous cell carcinoma and its relationship with patient prognosis
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摘要:目的 探讨Erbin在食管鳞状细胞癌(esophageal squamous cell carcinoma, ESCC)中的表达意义及其与患者预后的关系。方法 利用免疫组织化学法检测含有299例食管癌病例的组织芯片中Erbin的表达情况, 并分析Erbin的表达与临床病理参数和患者生存时间的关系, 采用Cox回归预测各临床病理参数的危险度。此外, 借助蛋白印迹和PCR方法检测25例冷冻的食管癌组织和癌旁正常组织中Erbin的蛋白和基因表达。结果 与癌旁正常食管上皮相比, Erbin蛋白和mRNA在ESCC组织中的表达显著增高(55.2%vs.0, P< 0.05), 且Erbin的高表达与患者的TNM分期(64.9%vs.47.3%, P=0.002)和淋巴结转移状态密切相关(65.5%vs. 45.0%, P< 0.001), Erbin的高表达也与患者的不良预后密切相关(P< 0.05)。结论 Erbin在食管鳞状细胞癌中表达增高并与患者的不良预后密切相关, 提示Erbin可能是一个重要的肿瘤患者预后的生物标记物。Abstract:Objective To explore the significance of Erbin expression in esophageal squamous cell carcinoma (ESCC) and its relationship with patient prognosis.Methods Erbin expression in a tissue chip containing samples from 299 cases were examined using immunohistochemistry; in addition, the relationship between Erbin expression and clinical-pathological parameters and patient survival time were also analyzed.Cox regression was used to predict the risk of clinical-pathological parameters.The mRNA and protein expression of Erbin was also determined in 25 cases with paired ESCC and normal tissues through polymerase chain reaction (PCR) and Western blot.Results The expression of Erbin protein and mRNA in ESCC were significantly higher than those of normal esophageal epithelium adjacent to cancer (55.2%vs.0, P< 0.05).The high expression of Erbin in ESCC was closely related to TNM stage (64.9%vs.47.3%, P=0.002) and lymph node metastasis (65.5%vs.45.0%, P< 0.001).Moreover, the high expression of Erbin in ESCC was closely related to poor prognosis (P< 0.05).Conclusions Erbin expression was increased in ESCC and was closely related to poor patient prognosis, which suggested that Erbin may be an important biomarker for the prognosis of cancer patients.
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