低剂量紫杉醇联合沙利度胺抑制小鼠S-180肉瘤血管生成的研究

Low-dose paclitaxel combined with thalidomide inhibits angiogenesis in mice bearing S180 sarcoma

    摘要
  • 摘要:
      目的  探讨低剂量紫杉醇联合沙利度胺对小鼠S-180肉瘤血管生成的作用。
      方法  建立S-180移植瘤小鼠模型，将40只荷瘤小鼠随机分为4组。A组：生理盐水组（对照组）；B组：紫杉醇组，腹腔内注射20 mg/kg，每周3次，连续2周；C组：沙利度胺组，200 mg/kg灌胃，每周3次，连续2周；D组：紫杉醇+沙利度胺组，腹腔内注射紫杉醇（20 mg/kg）和沙利度胺（200 mg/kg）灌胃，每周3次，连续2周。观察瘤重、抑瘤率、血管内皮生长因子（vascular endothelial growth factor，VEGF）的表达和微血管密度（microvessel density，MVD）计数。
      结果  与对照组比较，B、C、D组均能降低瘤重、减少VEGF的表达、降低MVD计数（P < 0.05）；D组分别与B、C组比较，VEGF的表达及MVD计数均低于B、C组，且差异具有统计学意义（P < 0.05）。
      结论  小剂量紫杉醇联合沙利度胺能一定程度上抑制S180肉瘤血管的生长，两者具有协同作用。

     

    Abstract:
      Objective  To investigate the effect of low-dose paclitaxel combined with thalidomide on angiogenesis in mice bearing S180 sarcoma.
      Methods  An S180 sarcoma-bearing mouse model was established. Forty tumor-bearing mice were randomly divided into four groups:A, the control group, administered normal saline; B, the paclitaxel group, administered 20 mg/kg paclitaxel by intraperitoneal injection three times per week for 2 weeks; C, the thalidomide group, administered 200 mg/kg thalidomide by gavage three times per week for 2 weeks; and D, the paclitaxel + thalidomide group, administered paclitaxel (20 mg/kg) and thalidomide (200 mg/kg) by intraperitoneal/intragastric administration three times per week for 2 weeks. Tumor weight, tumor inhibition rate, microvascular density (MVD), and vascular endothelial growth factor (VEGF) expression were measured.
      Results  Tumor weight, VEGF expression, and MVD were lower in groups B, C, and D (P < 0.05) than in the control. VEGF expression and MVD were lower in group D than in groups B and C; these differences were statistically significant (P < 0.05).
      Conclusions  Low-dose paclitaxel combined with thalidomide exerted an inhibitory effect on vascular growth in S180 sarcoma.

     

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