Abstract: Objective: To establish a murine uterine cervical cancer cell line and to define its biological character-istics. Methods: Transplanted tumor tissues were used for primary culture of U14 cervical carcinoma cells in vitro. After20 passages, we detected CK expression with immunohistochemistry, observed cell cycle with flow cytometry, analyzed thechromosomes, determined the cell growth curve, and checked tumorigenicity and metastatic potential. We also transfectedU14 cells with the plasmid pEGFP-N1. Results: The newly established murine cell line was continuously passaged 50times in 10 months. The cells showed positive CK expression. The karyotype was hypotetraploid, and the modal numberwas 64-68. The cell doubling time was 21.83 hours, and the cell line shows exponential growth on the third and fourthdays. Cell cycle analysis showed that 34% of the cells were in G₁ phase, 26.4% of the cells were in G₂ phase, and 39.6%of the cells were in S phase. After implantation, tumorigenicity was 100%. No mycoplasma was detected. We also estab-lished a monoclonal continuous cell strain named U14-GFP with 100% positive expression of GFP. Conclusion: We suc-cessfully established a new murine cervical carcinoma cell line named U14 and a related cell strain U14-GFP that ex-presses GFP. Both strains can be used for research in vivo or in vitro.