Abstract: Objective: To explore the safety and feasibility of Elemene combined with continuous hyperthermic hypotonic peri-toneal perfusion(CHHPP) in rabbits. Methods: A total of 24 New Zealand rabbits were randomly divided into four groups.Group A1 received Elemene-CHHPP(n=6), group A2 received Cisplatin-CHHPP(n=6), group A3 received the same dose of Elemene combined with Cisplatin-CHHPP (n=6), and group A4 received only CHHPP (n=6). Routine blood work, liver function tests, renal function tests and quantitation of CD4 and CD8 T-lymphocytes in the peripheral blood were per-formed before and after the treatment. All of the experimental rabbits were sacrificed on the eighth day after treatment. The condition of the abdominal wound and the abdominal cavity adhesions were observed using light microscopy. Tissue samples from the liver and the kidney were histologically examined with light microscopy. Results: The white blood cell count(WBC) increased significantly after the treatment in group A1(P<0.05). Myelosuppression was observed in group A2(P<0.05), and the WBC in group A2 was significantly lower than that in the other three groups (P<0.01, P<0.01, and P<0.01, respectively). The serum level of globulin(GLB) was significantly increased and the albumin (ALB) was decreased in all 4 groups (P<0.05 and P<0.01, respectively). The blood aspartate (AST) level in group A2 was significantly increased compared with that of the other three groups. The blood urea nitrogen (BUN) in group A2 and group A3 was significantly increased compared with that of group A1 and group A4. No significant difference was found in the histomorphology a-mong the 4 groups, but the liver samples from 2 rabbits in group A3 appeared to have sub-massive necrosis and massive necrosis. Conclusion: Elemene-CHHPP is feasible and safe. Elemene can decrease the side effects caused by CHHPP.