Abstract: Objective : To establish a T-24 human bladder cancer cell line expressing IL-18 protein and to explore the effect of exogenous IL-18 on the growth and biological behavior of T-24 cells. Methods : A plasmid containing the IL-18 gene was sequentially transfected into two packing cell lines (ecotropic ψ2 and amphotropic PA317) using the LXSN retrovirus.The PA317-positive cell clones that expressed IL-18 were selected using G418.T-24 cells were then transfected with IL-18/PA317 and IL-18-expressing T-24 cells were selected.RT-PCR, ELISA, and immunohistochemistry were used to detect the expression of IL-18 mRNA and protein.IL-18-ex-pressing T-24 cells were screened for clones with high expression of IL-18.Changes in cell cycle and apopto-sis were observed with flow cytometry before and after transfection.The ability of mouse splenocytes to se-crete IFN-γ induced by culturing with supernatant from IL-18-expressing T-24 cells was detected with ELISA.Proliferation and adhesion were detected by MTT assay before and after transfection. Results : The IL-18-transfected T-24 cells showed stable expression of IL-18 mRNA and protein.Culturing mouse spleno-cytes with supernatant from IL-18-expressing T-24 cells significantly promoted secretion of IFN-γ ( P <0.01).The cell cycle and apoptosis rate were not significantly different among the IL-18-expressing T-24 cells, LXSN/ T-24 cells, and T-24 cells. Conclusion : The T-24 cellular clones stably expressing IL-18 maintain tumor im-munogenicity and secrete IL-18, which evokes an immune response.IL-18-expressing T-24 cells can be used to research gene therapies for bladder cancer.