SFRP1基因表达与大肠肿瘤发生的关系研究
The Relationship between SFRP1 Expression and Colorectal Tumorigenesis
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摘要: 目的: 探讨SFRP1与大肠癌发生的关系。 方法: 运用半定量RT-PCR方法及免疫组化方法检测SFRP1、β-catenin、E-cad在正常大肠粘膜、大肠腺瘤以及大肠腺癌中的表达。 结果: 大肠腺癌、腺瘤组织的β-catenin、E-cad胞膜表达不同程度缺失,β-catenin呈胞质和胞核异位表达,腺瘤β-catenin的异位表达率为87.5%,低于大肠腺癌(100%,P<0.05)。大肠腺瘤β-catenin、E-cad胞膜表达缺失率分别为37.5%、37.5%,低于大肠腺癌(58.8%、76.4%,P均<0.05)。半定量RT-PCR显示,SFRP1基因在大肠腺瘤、大肠腺癌组织中的表达水平均明显低于大肠正常组织的对照组,且大肠腺癌组织中SFRP1基因的表达水平低于大肠腺瘤(P<0.05)。 结论: SFRP1基因下调使其拮抗Wnt途径的功能削弱,导致Wnt的持续存在,其下游基因β-catenin、E-cad异常表达,从而导致大肠粘膜细胞获得恶变潜能。故SFRP1可能为预防大肠肿瘤的发生、大肠肿瘤的早期诊断提供新思路,并为防治大肠肿瘤提供新靶标和实验依据。Abstract: Objective: To investigate the relationship between SFRP1 gene expression and colorectal tumorigenesis. Methods: We employed immunohistochemical staining and semiquantitative reverse transcriptionpolymerase chain reac-tion(RTPCR) to detect the expression of SFRP1, β catenin, and Ecad genes in normal colorectal mucosa, colorectaladenoma and colorectal cancer samples. Results: Abnormal expression of β catenin and Ecad were found in colorectaladenoma samples and colorectal cancer samples. SFRP1 expression was downregulated in colorectal adenoma samples andcolorectal cancer samples. Conclusion: Downregulation of SFRP1 decreases its antagonism of the Wnt pathway, leading toabnormal expressd:\PDF\.pdfion of β catenin and Ecad and an increase in the potential for colorectal tumorigenesis. SFRP1 may bea target for prevention and treatment of colorectal tumors.
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