应用RT-PCR检测CK19表达提高乳腺癌前哨淋巴结微转移检出的研究

顾林; 冯玉梅; 潘乐康; 王立梅; 方志沂

应用RT-PCR检测CK19表达提高乳腺癌前哨淋巴结微转移检出的研究

天津医科大学附属肿瘤医院乳腺癌防治中心天津市 300060

详细信息

中图分类号: R737.9
计量
- 文章访问数: 2
- HTML全文浏览量: 0
- PDF下载量: 2
出版历程
- 修回日期: 2003-11-27
- 发布日期: 2004-02-14

Detection of Micrometastasis in Sentinel Lymph Nodes of Breast Cancer Using CK19 RT-PCR

Center for Breast Cancer, Cancer Hospital of Tianjin Medical University, Tianjin

摘要

摘要: 目的:评价乳腺癌前哨淋巴结活检的临床意义,应用细胞角蛋白cytokeratin19(CK19)RT-PCR检测法提高其的敏感性及准确率。方法:随机选取1999年10月至2003年3月住院的乳腺癌患者73例,其中2001年1月至2003年3月的29例,对前哨淋巴结(SLN)进行了CK19RT-PCR检测。采用专利兰进行SLN识别。RT-PCR法检测CK19mRNA表达。结果:SLN识别成功67例,成功率为91.8%(67/73)。对上述67例的SLN转移的常规病理诊断的敏感度68.8%(22/32),准确率85.1%(57/67)。在行CK19RT-PCR的29例中,检测出常规病理未检出的微小转移病例8例。敏感度90.0%(18/20),准确率93.1%(27/29)。结论:前哨淋巴结活检可有效判断乳腺癌腋淋巴结转移状态,应用CK19RT-PCR检测SLN的微转移,可提高敏感度及准确率。
- 乳腺癌 /
- 前哨淋巴结 /
- cytokeratin19 /
- RT-PCR
Abstract: Objective :To evaluate the significance of sentinel lymph node biopsy (SLNB)in breast carcinoma and to improve the sensitivity and accuracy of SLNB by cytokeratinl9 ( CK19)RT-PCR. Meth-ods :Unselected 73 patients with breast carcinoma underwent SLNB using patentblue, and SLNs of 29 patients were detected with CK19 RT-PCR. Results : SLNs were identifiedin 67 patients (91.8%). The sensitivity and accuracy of routine examination of pathology for SLNswas 68.8%C22/32)and 85.1%(57/67) respectively. The sensitivity and accuracy of CK19 RT-PCRfor SLNs was 90.0%C 18/20)and 93.1%(27/29) respectively. Conclusion : SLNB is useful to dleter-mine the status of axillary lymph nodes of breast carcinoma.CK19 RT-PCR can improve thesensitivity and accuracy of SLNB.
- Breast carcioma /
- Sentinel lymph node /
- Cytokeratin19 /
- RT-PCR

HTML全文

参考文献(1)

[1]

1. Veronesi U, Paganelli G, Galimberti V, et al. Sentinel nodebiopsy to avoid axillary dissection in breast cancer with clinitally negative lymph nodes[J].Lancet, 1997, 349(9069): 1864-1867
2. Ishida M, Kitamura K, Kinoshita J, et al Detection of mi-crometastasis in the sentinel lymph nodes in breast cancer[J] Surgery, 2002, 131(1 suppl):S211-216
3. Matsumoto M, Natsugoe S, Ishigami S, et al. Lymph nodemicrometastasis and lymphatic mapping determined by reversetranscriptase-polymerase chain reaction in pN0 gastric carcinoma[J]Surgery, 2002, 131(6):630-635
4. Yoshioka S, Fujiwara Y, Sugita Y, et al. Real-time rapid reverse transcriptase-polymerase chain reaction for intraoperativediagnosis of lymph node micrometastasis: Clinical applicationfor cervical lymph node dissection in esophageal cancers[J]Surgery, 2002, 132(1):34-40
5. 冯玉梅, 郝希山, 于泳, 等. CK19作为微转移癌检测基因标志的假基因干扰及解决力法田.中华肿瘤杂志, 2001,23(4):330-331
6. Albertini JJ, Lyman GH, Gox G, et al. Lymphatic mappingand sentinel lymph node biopsy in the patients with breastcancer[J].JAMA, 1996, 276(22):1818-1822
7. Giuliano AE, Jones RG, Brennan M, et al. Sentinel lymphadenectomy in breast cancer [J]. J Glin Oncol, 1997, 15(6):2345-2350
8. Giuliano AE, Kirgan DM, Guenther JM, et al. Lymphaticmapping and sentinel lymphadectomy for breast cancer[J].Ann Surg, 1994, 220(3):391-401
9. Rosen PP, Lesser ML, Kinne DM, et al. Discontinuous orskip metastasis in breast carcinoma: analysis of 1228 axillarydissections[J].Ann Surg, 1983, 197(3):276-283
10. Van Lancker M, Goor G, Sacre R, et al. Patterns of axillarylymph node metastasis in breast cancerJ」.Am J Glin Oncol,1995, 18(3):267-272

施引文献

资源附件(0)

计量

文章访问数: 2
HTML全文浏览量: 0
PDF下载量: 2
被引次数: 0

应用RT-PCR检测CK19表达提高乳腺癌前哨淋巴结微转移检出的研究

计量

出版历程

Detection of Micrometastasis in Sentinel Lymph Nodes of Breast Cancer Using CK19 RT-PCR

计量

出版历程

目录