胃癌线粒体DNA拷贝量降低一种新的肿瘤标志物?

韩波, 李凡, 杨雪飞, 辛彦

韩波, 李凡, 杨雪飞, 辛彦. 胃癌线粒体DNA拷贝量降低一种新的肿瘤标志物?[J]. 中国肿瘤临床, 2004, 31(5): 244-246.
引用本文: 韩波, 李凡, 杨雪飞, 辛彦. 胃癌线粒体DNA拷贝量降低一种新的肿瘤标志物?[J]. 中国肿瘤临床, 2004, 31(5): 244-246.
Han Chengbo, Li Fan, Yang Xuefei, . Decreased mtDNA Copy Number of Gastric Cancer:A New Tumor Marker?[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2004, 31(5): 244-246.
Citation: Han Chengbo, Li Fan, Yang Xuefei, . Decreased mtDNA Copy Number of Gastric Cancer:A New Tumor Marker?[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2004, 31(5): 244-246.

胃癌线粒体DNA拷贝量降低一种新的肿瘤标志物?

详细信息
  • 中图分类号: R730.231

Decreased mtDNA Copy Number of Gastric Cancer:A New Tumor Marker?

  • 摘要: 目的:比较线粒体基因组(mitochondrialDNA,mtDNA)拷贝数在胃癌和癌旁正常组织间的差异,探究mtDNA与胃癌发生的关系。方法:PCR分别扩增胃癌组织和癌旁正常粘膜组织各20例共40个样本的线粒体D-loop区两个高变区HV1(Hypervariableregion)和HV2;并以核基因组的β-actin作为定量标准物。聚丙烯酰胺凝胶电泳(Polyacrylamidegelelectrophoresis,PAGE)银染比较mtDNA拷贝数在癌和正常组织间的差异。结果:HV1和HV2拷贝量(用β-actin标准化)在胃癌组织和胃正常组织间有显著的差异,P>0.01;其拷贝量与环腺苷酸磷酸二酯酶(cAMP-PDE)和环鸟苷酸磷酸二酯酶(cGMP-PDE)表达有统计学联系,P>0.05。结论:胃癌发生与mtDNA拷贝量的减少有着密切的关系,可能成为一种新的肿瘤分子标志物。
    Abstract: Objective : The aim is to explore the relationship between mtDNA (mitochondrialDNA) and gastric cancer by comparing the difference of mtDNA copy number in gastric cancers andpara-cancerous normal tissues. Methods :HV1, (Hypervariable reigon) and HVZ of mitochondrial D-loop region from 20 gastric cancers and 20 para-cancerous normal tissues were amplified via PCR,meantimep-actin was served as a quantitative standard marker, followed by Polyacrylamide gel elec-trophoresis (PAGE) and silver staining, which compared the difference of mtDNA copy number betweengastric cancers and normal tissues. Results : There existed significantly quantitative difference in HV2,HVZ (standardized withp-actin) between gastric cancers and normal tissues (P<0.01). mtDNA copynumber was associated with cAMP-PDE and cGMP-PDE (P<0.05). Conclusion : Gastric carcinogene-sis was closely correlated with decreased mtDNA copy number, which would be a new tumor marker.
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出版历程
  • 收稿日期:  2003-08-19
  • 发布日期:  2004-03-14

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