Abstract: Objective : To investigate the effects of p57^kip2 and cyclinE proteins on the genesis andprogression of human pancreatic cancer. Methods : The expression of p57^kip2 and cyclin E proteins intumor tissues and adjacent tissues of pancreatic cancer in 32 patients were detected by SP immunohis-tochemical technique. Results : p57^kip2 protein positive-expression rate in tumor tissues of pancreaticcancer was 46.9%, which was lower than that in adjacent pancreatic tissue (X²=5.317, P<0.05), p57^kip2protein positive-expression correlated significantly with tumor cell differentiation (X²=4.979, P<0.05)and did not correlate significantly with lymph node metastasis (P>0.05); cyclin E positive-expressionrate in tumor tissues was 68.8%,which was higher than that in adjacent pancreatic tissues (X²=4.036, P<0.05); cyclin E positive -expression also correlated significantly with tumor cell differentiation andlymph node metastasis (X²=5.128; X²=4.693, P<0.05); cyclin E protein positive-expression rate in thetumor tissues of p57^kip2 protein positive-expression group was lower than that in p57^kip2 protein negative-expression group, there were no significant correlation between the two groups (r=-0.112 11, P>0.05). Conclusions : The decreased expression of p57^kip2 protein and/or over-expression of cyclin E proteinmay play an important role in the genesis and progression of pancreatic cancer.