Abstract: Objective : To investigate the cytotoxicity effect of Tumor-Specific Cytotoxicity T Lymphocytes (CTL) from peripheral blood and tumor-infiltrating lymphocytes (TIL) in patients with hepatocellular carcinoma activated by dendritic cells (DC) loaded with heptocellular carcinoma antigens on hepatocellular carcinoma. Methods : D C functional status was analyzed by their proliferational activities and detection of membrane molecules with indirect immune florescent and FCM, and cytotoxicity activities of Tumor -Specific Cytotoxicity T Lymphocytes and tumur -infiltrating lymphocytes activated by dentritic cells loaded with hepatocellular cancerous antigens were measured by MTT assay on hepatocellular carcinoma. Results : The expression of CDla, CD80, CD83 and H L A - D R molecules on the dentritic cells, Tumor-Specific Cytotoxicity T Lymphocytes and TIL cytotoxicity activities of patients with hepatocellular carcinoma were significantly lower than those of the nomal controls. Conclutions : Functional status of dentritic cells derived from peripheral blood of patients with hepatocellular carcinoma was inhibitory and cytotoxicity activities of Tumor-Specific Cytotoxicity T Lymphocytes and TIL activated by DC loaded with hepatocellular cancerous antigens. was significantly lower because this