Abstract: Objects : To observe the influence of different temperature on the apoptosis of human cervical cancer cell line (Hela cell). Methods : Hela cells were cultured with the common method and proceed with distinct temperature with water bath of warm-up method, and then the cell apoptosis was detected with flow cytometer and the status of D N A damage was analyzed with single cell gel electrophoresis after cultured for 24-hour continually. Results : The rate of cell apoptosis was increased with the raise of temperature and the maximum rate of cell apoptosis was 40.7% and 44.6% respectively after treated in 42.5(C and 43.5(C for one hour, and the rate of necrosis cell were 13.2% and 29.6%.The D N A damage was happened in 40% and 80% of cells which were treated for one hour in different temperature (42.5(C and 43.5(C), while there were only 20% of cells following this phenomenon. Conclusion: The cell apoptosis can be reduced by the warm-up method for one hour alone, and this can conducted the damage of DNA.