Abstract: Objective : To observe the effect of Granulocyte (recombinant human granulocyte colony-stimulating factor) and chemotherapy on autologous peripheral blood stem cells (APBSC) mobilization of patients with malignant solid tumors. Methods : This study includes 32 patients with solid tumor (malignant lymphoma 18, breast cancer 11 and germ cell tumors 3). The Granulocyte (Chugai Pharmaceutical Co, Ltd ) was given as a daily injecting subcutanously at the dose of 150~250μg body^-1 day^-1 from the day of the nadir of white blood cell (WBC) to the day before the end of APBSC harvest.APBSC apheresis was performed when WBC ≥5.0x10⁹ /L from the nadir and finished when accumulated MNC ≥5 x10⁸/kg or CD34⁺ cells count ≥2x10⁶/kg. Results : The median beginning time of Granulocyte given and apheresis was the day 12(5~15) and the day 16(13~20) from the begin of chemotherapy respectively. The median times of Granulocyte given was 5 (4~12). The total count of M N C and CD34⁺ cells harvested for each patient were 5.76 ±2.05 x10⁸/kg and 15.58 ±10.36x10⁶/kg, respectively. Colony forming ability of PBSC were done and the mean CFU-GM was 21.01±20.75x10⁴ /kg. A few side effects were observed in the mobilization. All 30 patients who underwent APBscT achieved hematopoiesis recovery. Conclusion : Granulocyte combining with chemotherapy was a safe and highly effective method for APBSC mobilization. The dose of 150<250μg body^-1 day^-1 of Granulocyt combining with chemotherapy were suitable for APBSC mobilization in Chinese patients.