Abstract: Objective : To evaluate the efficacy of Hydroxy-camptothecin (HCPT) plus Aureus Filtrate Preparation (Highly Agglutinative Staphylococcin, HASL) in combined therapy of patients with malignant relapsed and refractory pleural effusion. Methods : Sixty-six patients were involved, of which 36 patients were in the treatment group and 30 patients in the control group. All of the patients were drawn the liquid out of the chest cavity by putting the PICC and then different drugs were administered. In the treatment group, the HCPT 30mg and HASL 2 000~3 000U were administered to the pleural cavity and Dexamethasone 5 mg intromuscular injected, while the Cisplatin (CDDP) 60~70mg and Dexamethasone 10 mg were given to the pleural cavity and Metoclotramidum 20 mg intromuscular injected in the control group. Results : We got the response rate of 86.1％(31/36) with CR rate 55.6％(20/36) in the treatment group and the response rate of 63.3％(19/30) with CR rate 26.7％(8/30) in the control group. conclusion : The effect is increased significantly in the patients with relapsed and refractory malignant pleural effusion by using HCPT combined with HASL. With the advantage of easy handling, high response rate and also mild side effect, this method will sure be highly recommend in the future.