Abstract: Objective: To study the application value of chemotherapeutic hyperthermic intraperitoneal perfusion (CHIP) on treatment of ovarian carcinoma. Methods: The cells of peritoneal fluid were collected before and after CHIP and observed by electron microscopes in the operation of ovarian carcinoma. Results: Before CHIP, cells of peritoneal fluid were crowded under electron microscopes. Most of them were cancer cells. The structure of cancer cells was complete. There were microvillus on the surface of cytomembrane. The form of organelles was normal. After CHIP, cancer cells pieces or inflam -matory cells were seen clearly. The integrity of cancer cells was destroyed. The nucleus was degenerative solution. Mitochondrion vacuoles were changed. The form of organelles was abnormal, cytoplasm edema. Conclusion: CHIP could effectively kill the free cancer cell and it is of great significance for effectively preventing the postoperative metastasis and recurrence of ovarian carcinoma in abdominal cavity.