Role of Ginsenoside Rg3 in inducing Apoptosis of Tumor Cells in the Lymphatic Metastasis Mouse
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摘要: 目的 :观察人参皂甙Rg3对小鼠肝癌淋巴结转移模型中肿瘤细胞凋亡的诱导作用,探讨人参皂甙Rg3抗肿瘤淋巴结转移的机制。 方法 :建立小鼠肝癌淋巴结转移模型;光镜和透射电镜下观察各组(Rg3预防组、Rg3治疗组、Rg3与顺铂联合治疗组、顺铂治疗组及对照组)中原发瘤及转移瘤组织的形态学结构改变,并通过流式细胞仪分析肿瘤细胞的凋亡。 结果 :Rg3预防组及治疗组电镜下(5份样品)可见较多细胞凋亡小体的形成(5/5,4/5);顺铂治疗组的形态改变以细胞破坏为主,凋亡的细胞较少;联合治疗组细胞的凋亡和坏死程度相当。流式细胞学检测分析结果为:Rg3预防组、治疗组及联合治疗组均见细胞凋亡的特征峰(5/5),而顺铂治疗组为1/5,对照组未见凋亡峰(0/5)。 结论 :人参皂甙Rg3抗肿瘤细胞淋巴结转移的作用与诱导细胞凋亡有关。Abstract: Objective : To study the mechanism of Ginshenoside Rg3 in anti-lymphatic metastasis of hepatoma by the observation of the apoptosis effect which may be induced in mouse. Methods : The transplanted hepatoma models with lymphatic metastasis were set up, and the apoptosis of the primary and metastatic tumors was analysed in the following five groups: Ginsenoside Rg3 pretreatment, Ginsenoside Rg3 treatment, combination of Ginsenoside Rg3 & cisplatin treatment, cisplatin treatment and control (saline) groups. Results :The morphologic change under the light and electric miocroscope showed that more apoptosis bodies appeared in Ginsenoside Rg3 pretreatment and Ginsenoside Rg3 treatment groups compared with those of the other groups (5/5, 4/5); The extent of cell necrosis in cisplatin treatment group was more obvious; while the apoptosis and cell necrosis presenting in combination group in between. Apoptosis peak was observed by FCM and apoptosis nodule was detected in Ginsenoside Rg3-treated groups; The main change in the control group was cell injury, and no apoptosis peak detected. Conclusions : Apoptosis is one of the mechanisms of Ginsenoside Rg3 in volving its the anti-lymphatic, metastasis effect.
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Keywords:
- Ginsenoside Rg3 /
- Hepatoma /
- Lymphatic /
- Metastasis
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[1] 1 高船舟,曲淑贤,吕广艳,等.20(R)—人参皂甙Rg3对K562/ADM细胞凋亡诱导作用的研究[J].大连医科大学学报,2001,23(3):171~ 173
2 陈大富,赵扬冰,白绍槐,等.人参皂甙Rg3对乳腺浸润性导管癌的抗癌作用及其机制[J].四川大学学报,2003,34(3):546~548
3 寒川庆-,山下秀之,松田秀秋,等.高速液体クロマトゲラつイー しこよる 各种药用人参中サポニこの —齐分析[J].药学杂志,1995,115(3):241-249
4 Kitagaawa I, Akedo H, ShinJkai K, et al. Inhibition of tumor cell invasion and metastasis by ginsenosis Rg3[J]. Ginseng Rev, 1995, 16(1): 16-20
5 Shinkai K, Akedo H, Mukai M, et al. Inhibition of in vitro tumor cell invasion by ginsenoside Rg3 [J].JpnJ Cancer Res, 1996, 87(4): 357 ~362
6 赵翌,刘基巍.20(R)—人参皂甙Rg3抗肿瘤作用的研究进展[J]. 临床肿瘤学,2001,6(1):81~82
7 彭黎明,王曾礼,主编.细胞凋亡的基础与临床[M].北京:人民卫生出版社,2000.1~28
8 Kerr JER., Winterford CM, Harmon BV. Apoptosis; its significance in cancer and cancer therapy[J]. Cancer, 1994, 73 (8): 2013 ~ 2026
9 Venerando R, Miotto G,Pizzo P, et al. Mitochondrial alterations induced by aspirin in rat hepatocytes expressing mitochondrially targeted green fluorescent protein (mtGEP)[J]. EEBS Lett, 1996, 382(3): 256-260
10 朱海青,王振义.线粒体在细胞凋亡中的作用[J].国外医学分子生 物学分册,2000,22(1):27 ~30
11 凌茂英,刘希风,关素琴,等.小鼠肝癌为同转移力克隆的分离及其特性的研究[J].中华医学杂志,1990,70(6):315~318
12 侯力,凌茂英,吕申,等.两株不同转移能力小鼠肝癌细胞对宿主淋巴系统的影响[J] .实用肿瘤杂志,2000,15(2):90~92
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